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当前位置:首页> 产品选择指南 > 干细胞研究相关制品 > 细胞培养产品 (Cell culture) > Human Stem Cell Culture System (DEF-CS, all-in-one format)
Human Stem Cell Culture System (DEF-CS, all-in-one format)
品牌 Code No. 包装量 价格 说明书 数量
Cellartis Y30012 4 ml Inquiry
Cellartis Y30010 1 Kit 5,805 元
Cellartis Y30016 1 set Inquiry
Human iPS和Human ES增殖用培养基
(DEF-CS Culture System, all-in-one format)
作为一款高端创新型的产品,Cellartis DEF-CS培养系统使得单细胞干细胞操作成为常规的操作。该产品是专门针对人类诱导干细胞(hiPS)和人类胚胎干细胞(hES)研发的高效率增殖用培养基产品体系。该产品系统为all-in-one型,包括了所有组分,客户不需要另外求购组分。该产品是成分确定的培养基产品,而且不需要饲养层。DEF-CS既可以实现单细胞培养,也可以用于传统的iPS培养模式以及大规模干细胞增殖。在使用DEF-CS培养基增殖干细胞时,几乎没有背景分化的问题,这使得细胞筛选不再必需。作为一款创新产品,利用DEF-CS培养干细胞时,可以使用酶消化法(enzymatic passaging)实现需要单细胞操作,这一特点十分有利于高通量细胞鉴别筛选(high-throughput screening)、转染(transfection)、框架接种(scaffold seeding)等。
 
■ 产品特点
· Feeder-free and chemically defined culture system for efficient expansion of human iPS cells
· Ideal system for culturing iPS cells; cells maintain pluripotency and long-term potential for self-renewal
   and differentiation
· Complete kit includes 500 ml basal medium, additives, and coating compound
· Robust system provides high reproducibility and stable growth
· Maintains cells in an undifferentiated state with virtually no background differentiation
 
■ 产品应用
· Scale-up and mass production of human iPS cells
· Single-cell culture of human iPS cells
· Transfection and reprogramming
· High-throughput screening
· Tissue engineering (seeding cells on a scaffold)
 
Code No. Product Size
Y30012 Cellartis® DEF-CS 500 COAT-1 4 mL
Y30010 Cellartis® DEF-CS 500 Culture System 1 Kit
Y30016 Cellartis® DEF-CS 500 Additives 1 Kit
 
■ 产品详情
https://www.takarabio.com/products/stem-cell-research/media-and-supplements/pluripotent-stem-cell-media/cellartis-def-cs-500-culture-system
 
Robust growth of human induced pluripotent stem (iPS) cells in the Cellartis DEF-CS Culture System. The number of iPS cells was quantified after being cultured for three weeks using either the Cellartis DEF-CS Culture System, a reference feeder system, or four other stem cell culture systems.
 
参考文献:
1. Sivertsson, Louise, et al. "Hepatic differentiation and maturation of human embryonic stem cells cultured in a perfused
    three-dimensional bioreactor." Stem cells and development 22.4 (2012): 581-594.
2. Hanson, Charles, et al. "Transplantation of human embryonic stem cells onto a partially wounded human cornea in
    vitro." Acta ophthalmologica 91.2 (2013): 127-130.
3. Norrman, Karin, et al. "Distinct gene expression signatures in human embryonic stem cells differentiated towards
    definitive endoderm at single-cell level." Methods 59.1 (2013): 59-70.
4. Ulvestad, Maria, et al. "Drug metabolizing enzyme and transporter protein profiles of hepatocytes derived from human
    embryonic and induced pluripotent stem cells." Biochemical pharmacology 86.5 (2013): 691-702.
5. Ramirez JM, et al. Side scatter intensity is highly heterogeneous in undifferentiated pluripotent stem cells and
    predicts clonogenic self-renewal. Stem Cells Dev.2013 Jun 15;22(12):1851-1860.
6. Borestrom, Cecilia, et al. “Footprint-free human induced pluripotent stem cells from articular cartilage with
    redifferentiation capacity: A first step toward a clinical-grade cell source.” Stem Cells Trans. Med. (2014) 3, 433-447.
7. Kia, Richard, et al. "MicroRNA-122: a novel hepatocyte-enriched in vitro marker of drug-induced cellular toxicity."
    Toxicological Sciences (2014): kfu269.
8. Valton, Julien, et al. "Efficient strategies for TALEN-mediated genome editing in mammalian cell lines."
    Methods 69.2 (2014): 151-170.
9. Zandén, Carl, et al. "Stem cell responses to plasma surface modified electrospun polyurethane scaffolds."
    Nanomedicine: Nanotechnology, Biology and Medicine 10.5 (2014): 949-958.
10. Asplund, Annika, et al. “One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte
      Cultures Displaying? Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells” Stem Cell Rev and
      Rep
(2015)