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当前位置:首页> 产品选择指南 > 干细胞研究相关制品 > 细胞培养产品 (Cell culture) > Human Stem Cell Culture Medium (DEF-CS Xeno-Free)
Human Stem Cell Culture Medium (DEF-CS Xeno-Free)
品牌 Code No. 包装量 价格 说明书 数量
Cellartis Y30045 1 Kit 2,838 元
Cellartis Y30047 1 Kit 3,639 元
Cellartis Y30071 500 ml 5,911 元
临床前扩增人干细胞培养基系列(Xeno-free)
(The Xeno-Free Culture Media Product Family for Preclinical Expansion of Human iPS Cells)
Cellartis DEF-CS (Xeno-free) 培养基系列,完全无任何人源及动物源成分,被用于人类诱导多能干细胞 (iPSCs) 扩增。该款产品不使用饲养层 (feeder),而是使用xeno-free的基质物,比如 新型的iMatrix-511。使用DEF-CS 培养基 (xeno-free) 培养的细胞,拥有高的增殖率和稳定的核型,允许2D-单层培养和3D-悬浮培养进行有效扩增。
 
这些产品主要适用于
· 基础研究和临床前概念研究—Cellartis DEF-CS 500 Xeno-Free Culture Medium帮助您从基础研究过渡到下一阶段相关疾病模型的验证和生产制造战略的开展。
 
· 扩增到足够的细胞数—Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium搭配灌注生物反应器系统,提供了一个产生大量高质量的、未分化的、用作起始细胞治疗材料的理想方法。
 
· GMP 级生产和下游使用于临床研究—Cellartis DEF-CS 500 Xeno-Free GMP Grade Basal Medium (Prototype)遵循严格的生产准则,确保没有可能损害患者的人类和动物来源的物质。
 
■ 产品特点
Cellartis DEF-CS 500 Xeno-Free Culture Medium
· Maintains pluripotency and stable karyotype in a 2D non-colony monolayer culture
· Ideal for maintenance of undifferentiated pluripotent cells and scale-up to therapeutically relevant cell numbers
· Fully compatible with the GMP-grade version for a seamless transition to the clinic
Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium
· Pluripotent cells in the spheroids retain full differentiation potential and stable karyotype
· Produces 5 × 109 cells in a 1 L bioreactor within 3–4 passages
· Provides a distinct advantage for scale-up to therapeutically relevant cell numbers
Cellartis DEF-CS 500 Xeno-Free GMP Grade Basal Medium (Prototype)
· Optimized for small- or large-scale production of pluripotent cells for downstream use in clinical research settings
· Manufactured as a quality-assured product, according to the guidelines for GMP for investigational products
· Rigorous manufacturing standards ensure quality and consistency; all materials are of clinical quality, with traceable production processes
 
■ 产品应用
· Culturing human iPS cells in completely xeno-free conditions for preclinical studies.
 
■ 产品目录
Code No. Product Size
Y30045 Cellartis® DEF-CS 500 Xeno-Free Culture Medium w/o antibiotics 1 kit
Y30042 Cellartis® DEF-CS 500 Xeno-Free Additives 1 kit
Y30046 Cellartis® DEF-CS 500 Xeno-Free Basal Medium w/o antibiotics 500 ml
 
Code No. Product Size
Y30047 Cellartis® DEF-CS500 Xeno-Free 3D Spheroid Culture Medium w/o antibiotics 1 kit
Y30046 Cellartis® DEF-CS 500 Xeno-Free Basal Medium w/o antibiotics 500 ml
Y30048 Cellartis® DEF-CS500 Xeno-Free 3D Spheroid Additives 1 kit
 
Code No. Product Size
Y30071 Cellartis® DEF-CS 500 Xeno-Free GMP Grade Basal Medium (Prototype) 500 ml
 
■ 关联产品
Code No. Product Size
T303 iMatrix-511 2 × 175 μg
 
■ 产品详情
Y30045:https://www.takarabio.com/products/stem-cell-research/media-and-supplements/pluripotent-stem-cell-media/xeno-free-human-stem-cell-culture-medium
Y30047:https://www.takarabio.com/products/stem-cell-research/media-and-supplements/pluripotent-stem-cell-media/xeno-free-3d-spheroid-culture-medium
Y30071:https://www.takarabio.com/products/stem-cell-research/media-and-supplements/pluripotent-stem-cell-media/xeno-free-gmp-grade-stem-cell-culture-medium
 
High-quality hiPS cell aggregates grown with Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium in stirred tank bioreactors (in perfusion mode). hiPS cell aggregates grown under 4% O2 perfusion conditions were sequentially passaged three times by mechanical disruption (protocol adapted from Otsuji et al. 2014); cells were counted at passage on days 4 and 7, and at the final time point on day 11, to determine the expansion factor relative to the initial seeding number.
 
High-quality hiPS cell aggregates were grown with Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium in stirred tank bioreactors (in perfusion mode). After expansion of hiPS cells as aggregates, cells were harvested on day 4 and expression of OCT4, SSEA-4, TRA-1-60 (pluripotency markers) and SSEA-1 and SOX17 (differentiation markers) was analyzed by immunostaining.
 
  比较结果来源于TBUSA网站