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高保真
 
 Application  In-Fusion Cloning  Long PCR,
 sequencing, cloning
 Cloning and expression,
 DNA sequencing
 Recommended product  CloneAmp HiFi PCR
 预混合液
 PrimeSTAR GXL DNA
 Polymerase
 PrimeSTAR Max DNA
 Polymerase
 Amplicon size
 gDNA  ﹤6 kb (human),
 ﹤10 kb (E. coli)
 ﹤30 kb  ﹤6 kb
 Plasmid/lambda  ﹤15 kb  ﹤40 kb  ﹤15 kb
 cDNA  ﹤6 kb  ﹤13.5 kb  ﹤10 kb
 Enzyme properties
 5'→3' exonuclease
 activity
     
 3'→5' exonuclease
 activity
 √  √  √
 T/A overhangs or
 blunt
 Blunt  Blunt  Blunt
 Fidelity (sequencing)  29X Taq (GC-rich
 target)
 6.5X Taq (GC-rich
 target)
 29X Taq (GC-rich target)
 Speed  5 sec/kb  10 sec/kb (rapid
 protocol)
 5 sec/kb
 GC-rich targets    Up to 73%  
 Format/features
 Premix  √    √
 Hot start  √  √  √